Imaging the Activity of Immobilized Horse Radish Peroxidase with Scanning Electrochemical/chemiluminescence Microscopy

Scanning electrochemical microscopy. 44. Imaging of horseradish peroxidase immobilized on insulating substrates.

Scanning electrochemical microscopy (SECM) was used to study horseradish peroxidase (HRP) immobilized with copolymer on insulating substrates (glass slide or polycarbonate membrane filter). Two methods were used to immobilize HRP: In the first, HRP was coimmobilized by cross-linking on a glass slide with a copolymer swelled in water to form a hydrogel; in the second, the same copolymer and avid...

Imaging of localized enzymatic peroxidase activity over unbiased individual gold nanowires by scanning electrochemical microscopy

Scanning electrochemical microscopy (SECM) in the constant-height mode was used to image individual gold nanowires (AuNWs, 2–3 mm long and 140 nm diameter). High-resolution negative and positive feedback current images of individual AuNWs immobilized on glass and gold-coated glass slides, respectively, were recorded with a Wollaston-based platinum disk ultramicroelectrode (UME) of radius 300 nm...

Purification of horse-radish peroxidase and comparison of its properties with those of catalase and methaemoglobin.

ABSORPTION OF THE HORSE-RADISH PEROXIDASE IN THE SMALL INTESTINE OF CmCKEN

Holman J.: Absorption of the Horse-radish Peroxidase in the Small Intestine of Chicken. Acta vet. Brno, 47, 1978: 3-6. Localization of the horse-radish peroxidase was studied in the epithelium and stroma of the small intestitlal villi in the chic:, aged 37 days. The enzyme (molecular weight 40.000) was injected into the ligated jejunal loop. Its enteral absorption was shown to proceed via the s...

Fabricating and imaging carbon-fiber immobilized enzyme ultramicroelectrodes with scanning electrochemical microscopy.

The scanning electrochemical microscope (SECM) is used to image the activity of enzymes immobilized on the surfaces of disk-shaped carbon-fiber electrodes. SECM was used to map the concentration of enzymatically produced hydroquinone or hydrogen peroxide at the surface of a 33-microm diameter disk-shaped carbon-fiber electrode modified by an immobilized glucose-oxidase layer. Sub-monolayer cove...